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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: J Immunol. 2008 Oct 1;181(7):4471–4484. doi: 10.4049/jimmunol.181.7.4471

FIGURE 3.

FIGURE 3

The induction of inhibitory γ9δ2 T cells does not require viable mycobacteria or involve known TLR ligands expressed by mycobacteria. A, PBMC were either rested in medium or stimulated with live BCG or soluble M. tuberculosis (Mtb) lysates for 7 days. The expanded γδ T cells were immunomagnetically purified and then co-cultured at a 10:1 E:T ratio with BCG-infected macrophages to determine inhibitory effects against intracellular mycobacteria. *p< 0.05 comparing the effects of BCG- or MtbWL-expanded γδ T cells with control cultures by Wilcoxon matched pairs tests (n=5/group). B, TLR co-stimulation during IPP expansion does not generate inhibitory γ9δ2 T cells. PBMC were expanded with the indicated concentrations of TLR agonists in the presence or absence of 10 μM IPP for 7 days. Shown are fold expansions of γ9δ2 T cells (Open bars, right axis) and % inhibition of intracellular mycobacteria (Filled bars, left axis).