FIGURE 6.

High correlations between Sequencing and Spectratyping data rule out artifactual in vitro skewing during the cloning techniques used to obtain sequencing results. Continuous variables for comparative analysis were generated for both sequencing and spectratyping data by multiplying the nucleotide size of each fragment times its observed frequency within the population. Spearman-rank correlations were analyzed between these nt size * frequency products for each individual’s paired data as described in Experimental Procedures. R² coefficients were 0.704, 0.983, and 0.951 for all three different volunteers (all p < 0.0001 by Spearman-rank tests).