Table 1.

Bumetanide and furosemide do not affect secondary axis-inducing activity of NKCC1

	NAM	NAM + bumetanide (0.9 mm)	NAM + furosemide (2 mm)
Uninjected controls	0% (n= 67)	0% (n= 61)	0% (n= 47)
FL NKCC1	32% (n= 59)	36% (n= 61)	29% (n= 55)
1079–1166 NKCC1	32% (n= 62)	35% (n= 65)	32% (n= 60)

Four nanograms of 1079–1166 NKCC1 or 800 pg of FL NKCC1 mRNAs were injected in one ventral blastomere at the 4-cell stage, and the embryos were scored for secondary axes 2.5 days later at stage 35. NAM, normal amphibian media. Furosemide and bumetanide were added to the media 2 h after injection (the same results were observed if the inhibitors were present from the time of injection).