Table 2.

Secondary axis-inducing activity of NKCC1 does not require Na⁺ or Cl⁻ ion flux

	MMR	MMR – Na+	MMR – Cl−
Uninjected controls	0% (n= 40)	0% (n= 36)	0% (n= 37)
FL NKCC1	36% (n= 30)	36% (n= 31)	30% (n= 30)
1079–1166 NKCC1	27% (n= 34)	24% (n= 33)	28% (n= 31)

MMR media with indicated modifications (see Methods) was used to incubate embryos injected with 4 ng of 1079–1166 NKCC1 or 800 pg of FL NKCC1 mRNAs in one ventral blastomere at the 4-cell stage, and the embryos were scored for secondary axes 2.5 days later at stage 35.