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. 2008 Dec 15;587(Pt 3):625–640. doi: 10.1113/jphysiol.2008.165217

Figure 1. Relative abundance of Kv and KCa channels in mesenteric (A) and aortic VSMCs (B).

Figure 1

Expression levels are normalized with respect to RP18s. Relative abundance was expressed as Inline graphic, where ΔCt=Ct,channelCt,18s. The plots also show differences in K+ channels expression in BPH arteries (using BPN expression levels as calibrator) in mesenteric (C) and aortic beds (D). The changes were calculated as Inline graphic, where ΔΔCtCt(BPH)–ΔCt(BPN), and represented in log scale, so that negative values mean decreased expression and positive values increased expression. Each bar is the mean of 10 determinations obtained in 5 assays by duplicate. All through the figures, *P < 0.05, **P < 0.01 and ***P < 0.001.