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. 2008 Dec 15;587(Pt 3):625–640. doi: 10.1113/jphysiol.2008.165217

Figure 7. Characterization of the expression of Kv6.3 protein in BPN and BPH mesenteric VSMCs.

Figure 7

A, the presence of Kv6.3 subunits in BPN and BPH mesenteric arteries was analysed by Western blot. Mouse brain was the positive control and immunoblot with β-actin providing the loading control. The figure is representative of 4 different experiments. B, the functional contribution of Kv6.3 subunits to Kv currents was explored by analysing the effect of the intracellular application of anti-Kv6.3 antibodies on the current amplitude. The graph shows the normalized time course of the current amplitude in BPN and BPH cells up to 25 min after the establishment of the whole-cell configuration. Data are means ±s.e.m. of 5–7 cells. The inset represents the average inhibition obtained at 20 min in BPN (black bar) and BPH cells (white bar). Representative traces from one BPN and one BPH cell at two different time points (5 and 20 min) are also shown. These records were obtained with the protocol described in the methods section.