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. 2008 Dec 8;587(Pt 3):641–654. doi: 10.1113/jphysiol.2008.162719

Figure 1. mRNA and protein expression of E-cadherin and β-catenin from intestinal EC measured by real-time PCR (A and B) and western immunoblots (C–E).

Figure 1

Figure 1

A and B, results are expressed as the mean fold increase in target mRNA relative to β-actin mRNA. Compared with controls, mRNA expression of E-cadherin (A) and β-catenin (B) was significantly decreased after 7 days of TPN administration. C, representative gels for both E-cadherin and β-catenin. D and E, results of E-cadherin and β-catenin protein expression are shown. Both factors decreased significantly with TPN administration compared with controls. Note that glutamine administration partially prevented the TPN-associated decline in β-catenin expression; however, glutamine did not affect E-cadherin expression at either 3 or 7 days. *P < 0.01, **P < 0.001, #P < 0.05. Abbreviations: TPN, total parenteral nutrition; n= 5–6 for each group; **P < 0.001.