Figure 8. TPN administration led to a decreased expression of several down-stream tcf-4 transcription genes.

Cyclin D1 (A), c-myc (B) and Axin2 (C) expression significantly decreased after 7 days of TPN. For c-myc and cyclin D1, this decline was noted as early as 3 days after TPN administration. Glutamine supplemented TPN prevented the observed decline for cyclin D1 and Axin2 expression. *P < 0.01, **P < 0.001, #P < 0.05; n= 4–6 per group.