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. 2008 Dec 1;587(Pt 3):655–668. doi: 10.1113/jphysiol.2008.164947

Figure 2. Immunoblotting confirms that peroxiredoxin-6 is markedly up-regulated in the EDL of nNOS-knockout mice.

Figure 2

A, after SDS-PAGE, immunoblotting with anti-peroxiredoxin-6 antibodies revealed a single band (molecular mass 25 kDa) in the EDL extracts of nNOS-knockout mice. This band was only slightly detectable in the EDL extracts but prominent in lung extracts of C57 control mice. B and C, after 2D-PAGE, immunoblotting with anti-peroxiredoxin-6 antibodies demonstrated the presence of a 25 kDa protein with a pI of 6.5 in the EDL extracts of C57 control mice (B) and with pI of 5.9 in the EDL extracts of nNOS-knockout-mice (C). The corresponding areas (molecular mass of 25 kDa and pI of 5.9 or 6.5) in B and C are encircled.