Figure 3. Quantitative immunoblotting analysis.

Fifty micrograms of protein derived from the EDL homogenates of C57 control mice and nNOS-knockout mice were subjected to immunoblotting with antibodies against four proteins being present in the 2D-PAGE analysis at higher concentrations in the EDL of nNOS-knockout mice than in that of C57 control mice and against eNOS. The levels of peroxiredoxin-3, SOD1 and HSP25 were higher in the EDL of nNOS-knockout-mice than in that of C57 control mice, while those of prohibitin and eNOS (determined also in the vascular endothelial cell fraction) did not vary between the two strains. As loading control, a nitrocellulose matrix stained with ponceau red is shown. Representative examples of n= 4 immunoblots for each protein and mouse strain.