Skip to main content
. 2008 Dec 1;587(Pt 3):655–668. doi: 10.1113/jphysiol.2008.164947

Figure 4. The peroxiredoxin-6-dependent hydrogen peroxide-degrading activity of the EDL is higher in nNOS-knockout than in C57 control mice.

Figure 4

The oxidation of xanthine by xanthine oxidase generates hydrogen peroxide, which oxidizes the non-fluorescent DCFH to the fluorescent DCF. The fluorescence signal emitted at a wavelength of 525 nm was measured after a 5 min incubation period at 37°C. The assay was performed in the presence of 50 μg aliquots of bovine serum albumin (1: control) and of proteins from EDL homogenates that were derived from C57 control (2) and nNOS-knockout mice (3). Prior to the assay, some of the EDL homogenates were subjected to immunoprecipitation with antibodies against peroxiredoxin-6 (4: C57 control; 5: nNOS-knockout) or a pre-immune serum (6: C57 control; 7: nNOS-knockout). Values represent mean ± standard deviation of 6 independent experiments. *Values in nNOS-knockout mice differ significantly from values in C57 control mice. #Values differ significantly from values of controls with BSA.