Figure 6. The production of free radicals but not of hydrogen peroxide is higher in the EDL of nNOS-knockout mice than in that of C57 control mice.

A, to quantify the concentration of free radicals, EDL homogenates from C57 control mice and nNOS-knockout mice were subjected to electron spin resonance (ESR) spectrometry using 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine (CMH) as spin probe. Mean values for 8 independent experiments are represented together with the standard deviation. *Values in nNOS-knockout mice differ significantly from those in C57 control mice. B, 200 μg aliquots of bovine serum albumin (BSA) and of EDL homogenates that were derived from C57 control and nNOS-knockout mice were incubated with DCFH at 37°C. The fluorescence signal emitted at a wavelength of 525 nm was monitored for 25 min incubation. Mean values for three independent experiments are represented together with the standard deviation.