Table 1.
Proteins whose expression levels were higher in the EDL of nNOS-knockout mice than in that of C57Bl/6 control mice as determined by 2D-PAGE
| Spot | Estimated mol. mass (kDa) | Estimated pI | Protein | Swissprot accession number | Sequence coverage | Theoretical mol. mass (kDa) | Theoretical pI | Fold change in expression nNOS-knockout/C57 control | P |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 30 | 5.4 | Prohibitin | P67778 | 24.3% | 30 | 5.6 | 2.0 | 0.002 |
| 6 peptides | |||||||||
| 2 | 25 | 5.9 | Peroxiredoxin 6 | O08709 | 52.5% | 25 | 5.7 | Not quantifiable | < 0.0001 |
| 8 peptides | |||||||||
| 3 | 22 | 6.1 | Peroxiredoxin 3 | P20108 | 27.8% | 22 | 5.7 | 1.9 | 0.030 |
| 5 peptides | |||||||||
| 4 | 16 | 6.4 | Cu2+/Zn2+-superoxide dismutase (SOD1) | P08228 | 32.7% | 16 | 6.0 | 1.9 | 0.047 |
| 4 peptides | |||||||||
| 5 | 23 | 6.4 | Heat-shock protein | P14602 | 33.9% | 23 | 6.1 | 1.7 | 0.007 |
| β-1 (HSP25) | 7 peptides | ||||||||
| 6 | 17 | 7.6 | Nucleoside diphosphate | Q01768 | 66.5% | 17 | 7.0 | 2.6 | 0.002 |
| kinase B (NDP kinase B) | 8 peptides |
The positions of the spots in the 2D-gels were established by comparison using protein markers of known molecular mass and pI. Quantitative expression data (‘Fold change’) were based on the densitometric analysis of gel duplicates of 5 animals of each strain. The 6 proteins were identified by LC-MS/MS. As peroxiredoxin-6 was not detectable in EDL of C57 control mice, its ‘fold exchange’ was not quantifiable.