Table 2.
Quantitative RT-PCR analysis for determination of mRNA concentrations of the six proteins being present at higher concentrations in the EDL of nNOS-knockout mice than in that of C57 control mice
| Copy no. in EDL (per μg of mRNA) |
||||
|---|---|---|---|---|
| Gene | C57 control | nNOS-knockout | Fold mRNA change* | P |
| Prohibitin | 141 633 ± 97 407 | 80 613 ± 68 178 | 0.57 | 0.424 |
| Peroxiredoxin-6 | 5 300 000 ± 2 671 573 | 22 640 000 ± 5 980 100 | 4.27 | 0.010 |
| Peroxiredoxin-3 | 1 396 333 ± 742 856 | 3 729 250 ± 1 664 531 | 2.67 | 0.049 |
| Cu2+/Zn2+-superoxide dismutase (SOD1) | 347 000 ± 122 233 | 656 250 ± 162 133 | 1.89 | 0.031 |
| Heat-shock protein β-1 (HSP25) | 400 167 ± 86 314 | 805 800 ± 490 732 | 2.01 | 0.026 |
| Nucleoside diphosphate kinase B (NDP kinase B) | 508 333 ± 138 564 | 968 500 ± 310 724 | 1.91 | 0.045 |
*
Fold change in mRNA expression nNOS-knockout/C57 control. The mRNA levels were quantified by RT-PCR. The copy numbers of each gene given per μg of RNA were determined using external standards. Mean values for 5 animals of each strain are represented together with the standard deviation.