Figure 2. Myostatin mRNA, LAP and mature protein in gastrocnemius muscles of male and female C57 mice from 2 to 32 weeks of age.

Values are mean (±s.e.m.) (n= 6 per group). Asterisks indicate significance at each time point (*P < 0.05, ***P < 0.001). A representative Western blot is shown for myostatin LAP and mature protein for male and female mice at each age. The abundance of actin is shown to demonstrate the similarity in loading of protein per lane. Note, there was low protein in the samples collected at 2 weeks and actin was not detected in the representative female sample at this age.