Table 2.
Primer sequences for real time PCR
| Gene | Forward primer | Reverse primer | PCR size (bp) |
|---|---|---|---|
| PRG | ACCGCCCTGAGATTACTTTTCTG | AGTTCTCTTTCCAGGTTCACCAC | 122 |
| GLUT2 | GTTGCTGGATAAGTTCACCTGGAT | GATTGGACCTGGCCCAATCT | 133 |
| GLUT5 | GGCCTCATCTTCCCATTCATTCAA | GGGTAGCAGGTGGGAGGTCATTA | 223 |
| SGLT1 | CTACATCCAGTCCATCACCCAGTTAC | CCAATCAGGAAGCCGAGAATCAG | 126 |
| Ghrelin | AGAGGCGCCAGCTAACAAGTAA | GCAGGAGAGTGCTGGGAGTT | 112 |
| PYY | AGCGGTATGGGAAAAGAGAAGTC | ACCACTGGTCCACACCTTCTG | 111 |
| Leptin | TCACACACGCAGTCGGTATCC | GTCTCGCAGGTTCTCCAGGTC | 183 |
| Amylin | CTGCCACTGCCCACTGAAAG | CACTTCCGTTTGTCCACCTGAG | 150 |
| FAS | GAGGACTTGGGTGCCGATTAC | GCTGTGGATGATGTTGATGATAGAC | 132 |
| ACC | CCTTCTTCTACTGGCGACTGAG | TAAGCCTTCACTGTGCCTTCC | 148 |
| GLK | CCGAGTGGCTTACAGTTCTG | ACCTGAGTGTTGGAGATGATTC | 140 |
| SREBP1c | TCATCAACAACCAAGACAGTG | AGAGAAGCAGGAGAAGAGAAG | 130 |
| SREBP2 | GTGATTGTCTTGAGCGTCTTC | CGGATAAGCAGGTCTGTAGG | 169 |
| HMGCR | GGGACCAACCTTCTACCTCAG | GACAACTCACCAGCCATCAC | 134 |
| CYP7A1 | GCTCTGGAGGGAGTGCCATTTAC | GCTGTGCGGATATTCAAGGATGC | 118 |
| LCAT | ATGGGTATGTGCGGGATG | CCAAGGCTGTGTCCAATAAG | 157 |
| IL-6 | CTCCGCAAGAGACTTCCAG | GGTCTGTTGTGGGTGGTATC | 120 |
| TNF-α | GTCGTAGCAAACCACCAAG | AGAGAACCTGGGAGTAGATAAG | 145 |
| UCP1 | GAGGTGGTGAAGGTCAGAATGC | GTCGTCCCTTTCCACAGTGTTG | 131 |
PRG, proglucagon; GLUT, glucose transporter; SGLT, sodium-dependent glucose transporter; PYY, peptide YY; FAS, fatty acid synthase; ACC, acetyl-CoA carboxylase; GLK, glucokinase; SREBP, sterol regulatory element binding protein; HMGCR, 3-hydroxy-3-methyl-glutaryl-CoA reductase; CYP, cholesterol 7 α-hydroxylase; LCAT, lecithin:cholesterol acyltransferase; IL-6, interleukin-6; TNF-α, tumour necrosis factor-α; UCP-1, uncoupling protein-1.