FIGURE 6.

c-Kit/D816V gains Src activity. Ba/F3 cells expressing wild-type (wt) or c-Kit/D816V were starved, stimulated with SCF (100 ng/ml), and subsequently immunoprecipitated using a c-Kit antibody. The immunoprecipitates were used in a kinase assay together with Src optimal peptide as substrate. The phosphorylation of the substrate by wild-type c-Kit and c-Kit/D816V was detected by Fuji FLA 3000, and signal intensity was quantified by the Multigauge software. Error bars indicate standard deviation.