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. 2009 Apr 24;284(17):11100–11109. doi: 10.1074/jbc.M806956200

FIGURE 5.

FIGURE 5.

Phosphorylation of Arr2 by recombinant Drosophila CaMKII1–275. A, CaMKII1–275 displays autonomous kinase activity. CaMKII1–275 was employed for the phosphorylation of GST fusion containing the autocamtide sequence with (lane 1) or without Ca2+/calmodulin (lane 3). CaMKII1–275 also phosphorylates fusion protein containing a short peptide of Arr2 encompassing Ser366 (lane 5). Substitution of Ser366 with Ala completely eliminates the phosphorylation (lane 6). Lanes 2 and 4 contain GST alone. B, CaMKII1–275 phosphorylates the C terminus of Arr2. GST fusion proteins containing the C terminus of Arr2 spanning residues 305–400 (∼41 kDa in size, arrow) were phosphorylated by recombinant CaMKII1–275 (lane 3). Note that this Arr2 fusion protein forms dimers. For comparison, phosphorylation of Arr2 peptide fusion is included in lane 1. In contrast, GST alone (lane 2) and fusion proteins containing the N terminus of INAD containing the first and second PDZ domains (∼74 kDa in size) were not phosphorylated (lane 4). Protein size markers are indicated on the left.