FIGURE 4.

FGF15 inhibits CYP7A1 and induces phosphorylation of FoxO1 in livers of mice. Mice were inoculated with the specified adenovirus (Ad) constructs and treated as described under “Experimental Procedures.” Total RNA was isolated and analyzed for CYP7A1 (A), PEPCK (B), or PGC-1α (C) gene expression. mRNA levels were analyzed from pooled RNA in each group (4–6 animals in each group) by q-PCR as described under “Experimental Procedures.” Results are expressed as -fold change relative to those of chow-fed mice infected with Ad-GFP, FGF15, or FoxO1 Δ256 as indicated. The mean values obtained from triplicates in each group are shown with error bars. Where pairwise comparisons provide p < 0.01, samples are labeled with a different symbol. D, protein extracts were prepared from pieces of the same mouse livers described in A–C. Pooled protein samples from animals in each group were analyzed by 8% SDS-PAGE and Western blotting with the indicated antibodies. P-FoxO1, phospho-FoxO1; T-FoxO1, total FoxO1; P-Akt, phospho-Akt, T-Akt, total Akt.