FIGURE 7.

FGF19 induces phosphorylation of FoxO1 in primary mouse hepatocytes. A, primary mouse hepatocytes were treated with 80 ng/ml FGF19 or 30 nm insulin for 30 min. Where indicated, primary mouse hepatocytes were treated with 100 nm wortmannin (W) or 20 μm SP600125 (S) for 30 min before treatment with FGF19. Total cell lysates were analyzed by 8% SDS-PAGE and Western blotting with an antibody against total-FoxO1 (T-FoxO1) or phospho-FoxO1 (P-FoxO1). B, primary mouse hepatocytes were treated with 80 ng/ml FGF19 for 30 min. Total cell lysates were analyzed as in A using an antibody against total-Akt (T-Akt) or phospho-Akt (P-Akt). C, total RNA was isolated from primary mouse hepatocytes treated with either wortmannin (100 nm) or FGF19 (80 ng/ml) alone or both, as indicated. Wortmannin was added at 30 min before the treatment of FGF19 for 6 h. mRNA levels were analyzed by q-PCR as described in the legend to Fig. 4.