FIGURE 8.

Fasting reduces FGFR4 mRNA and protein levels in mouse liver. Total RNA was isolated from liver and ileum of mice that were fed ad libitum a normal chow diet (Fed) or fasted for 24 h (Fast). mRNA levels were analyzed by q-PCR described under “Experimental Procedures.” Results are expressed as -fold change relative to those of the normal, and the mean values obtained from triplicates of pooled RNA from six animals in each group are shown with error bars. At the bottom, FGFR4 protein expression in fed and fasted mice was analyzed by immunoblotting with α-tubulin as control. *, p = 0.000005; **, p = 0.00008; #, p = 0.0077.