FIGURE 7.

HPLC analysis of the reaction products of DCS/CURS co-incubation. The front chromatograms show control reactions containing only CURS, and the back chromatograms indicate the reactions containing both DCS and CURS. Co-incubation of DCS and CURS in the presence of cinnamoyl-CoA (1a) and malonyl-CoA yielded a trace amount of dicinnamoylmethane (1e), whereas the control reaction containing only CURS did not (A). Co-incubation of DCS/CURS in the presence of p-coumaroyl-CoA (2a) (B) or feruloyl-CoA (3a) (C), in addition to malonyl-CoA, yielded a larger amount of bisdemethoxycurcumin (2e) or curcumin (3e), respectively, than the control incubation of only CURS (B and C).