FIGURE 7.
p38α, but not p38γ, is essential for oncogenic ras-induced increase in p16INK4A mRNA levels. Total RNA was isolated from BJ cells transduced with shGFP, shp38α-756, or shp38γ-550 and Ha-RasV12 (Ras) or vector (WH) on day 8 after transduction with Ras, separated on an agarose gel, transferred to nylon membrane, and hybridized to a human p16INK4A cDNA probe labeled by random priming. The signals were visualized and quantified with a PhosphorImager. The numbers represent the relative intensities of p16INK4A signals from Ras cells after being normalized to the signals from vector control cells.