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MgATP binding to the catalytic sites. MgATP binding to the three catalytic sites of the α3β3γ subcomplex of PS3 ATP synthase was measured in a buffer containing 50 mm Tris/H2SO4, 2.5 mm MgSO4, pH 8.0, at 23 °C (see “Experimental Procedures”). α3(βY341W)3γ, filled dots; Δ388GMDE391 in α3(βY341W)3γ, open squares; Δ392LSD394 inα3(βY341W)3γ, filled triangles. The lines are fitted binding curves based on the Kd values given in Table 5.
