TABLE 5.
Substrate | Reductase | k (s–1) | Yielda |
---|---|---|---|
Benzphetamine | Wild type | 0.15 ± 0.05 | 0.7 |
ΔTGEE | 0.1 ± 0.014 | 0.52 | |
Cyclohexane | Wild type | 2.5 ± 0.05 | 0.21 |
ΔTGEE | 3.3 ± 0.3 |
Expressed in units of nmol of product, norbenzphetamine or cyclohexanol, per nmol of cyt P450. The reactions were conducted as described under “Experimental Procedures”