FIGURE 3.

GLEPP1 inhibitors prevent cellular dephosphorylation of Syk and paxillin. A, evaluation of GLEPP1 inhibitors in human DHL10 lymphoma cells (39). Dox, doxycycline, used to induce expression of GLEPP1. BCR, B-cell receptor cross-linking using IgG and IgM to activate Syk kinase autophosphorylation. IP, immunoprecipitation. WB, Western blot immunostained using antibodies against Syk (α-Syk) or phosphotyrosine (α-PY). B, same as A but using human THP1 monocytes to measure steady-state, Compound 2-induced Syk phosphorylation. C, phosphorylation state of paxillin in BAC1.2F5 macrophages treated with GLEPP1 inhibitors (0 min) or following treatments with CSF1 for 15 min; averaged relative band density (top) and Western blot (bottom).