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Integrin binding activity correlates with kindlin-1 targeting to focal adhesions. Images of NIH 3T3 cells transiently transfected with GFP-tagged kindlin-1 wild-type and mutant expression constructs. Kindlin-1 co-localizes with vinculin at focal adhesions. Recruiting kindlin-1 to vinculin-rich focal adhesions is blocked by W612A but not Q612A mutations.
