TABLE 2.

Synthetic oligonucleotides used for mutagenesis of Leu⁴⁶⁵ to Ala, Lys, or Asp

Mutation	Oligonucleotide sequence	Restriction site
L465A	5′-GCCATCGCCTTCTCTGCGGGGTACCTCCTGGACAAG-3′	KpnI
L465K	5′-GCCATCGCCTTCTCTAAGGGGTACCTCCTGGACAAG-3′	KpnI
L465D	5′-GCCATCGCCTTCTCTGATGGGTACCTCCTGGACAAG-3′	KpnI