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Phosphorylation of MLC-20. Silver-stained glycerol-urea polyacrylamide gels of purified HMM II-C2 and HMM II-C1C2 are shown. In this system, the unphosphorylated (UnP) MLC20 migrates more slowly than phosphorylated (P) MLC20. The amount of HMM protein loaded is shown above each lane. HMM II-C2 and II-C1C2 were incubated in the presence (lanes 3, 4 and 7, 8) or absence (lanes 1, 2 and 5, 6) of MLCK.
