FIGURE 6.

Localization of NM II-C2-GFP in COS-7 cells. COS-7 cells were transfected with a plasmid encoding full-length NMHC II-C2-GFP or NMHC II-C0-GFP. Interphase (A) or dividing cells (B-D) are shown. Actin filaments were detected with rhodamine-labeled phalloidin (red). 4′, 6-Diamidino-2-phenylindole was used for nuclear staining. Note that NM II-C2-GFP localizes to filaments in interphase cells (A) and to the contractile ring in a dividing cell (C and D, arrow). NM II-C0-GFP also localizes to the contractile ring (B, arrow).