TABLE 2.

In vitro motility of HMM II-C with and without phosphorylation by MLCK

In vitro motility was measured at 30 °C as described under “Experimental Procedures.” The values are the mean and S.D. of three experiments from two different protein preparations. II-C0, noninserted HMM II-C; II-C1, inserted HMM II-C with an 8-amino acid insert in loop 1; II-C2, inserted HMM II-C with a 41-amino acid insert in loop 2; II-C1C2, inserted HMM II-C with both C1 insert in loop 1 and C2 insert in loop 2; NSM, no significant movement.

HMMs	In vitro motility
	−MLCK	+MLCK
	μm/s
II-C0	NSM	0.04 ± 0.005
II-C1	NSM	0.09 ± 0.004
II-C2	0.06 ± 0.001	0.06 ± 0.003
II-C1C2	0.06 ± 0.004	0.06 ± 0.003