FIGURE 3.

Cardiolipin acyl chain composition of wild-type and ygr110wΔ mutant strains. Mitochondria from wild-type BY4742 and ygr110wΔ mutant cells were isolated after 16 h of growth in YPD. Total lipids were extracted from mitochondria and separated by two-dimensional TLC as described under “Experimental Procedures.” Fatty acid (FA) profiles of CL were analyzed by GC-MS from ygr110wΔ (white bars) and wild-type (black bars). Data are expressed as relative percentage of total acyl chains in CL and represent means ± S.D. of at least three independent experiments. The ygr110wΔ mutant shows a 2.4-fold increased level of C16:0 acyl chains in CL compared with wild type.