Sequence analysis of TbPRMT7. A, ClustalW derived cladogram
of TbPRMT7 compared with the seven active human PRMTs as well as the
uncharacterized PRMT9(4q31) protein. The human PRMTs are grouped as Type I
(ADMA catalyzing), Type II (SDMA catalyzing), and the less definitively
characterized Type II or III (solely MMA catalyzing) PRMT7. TbPRMT7 is
indicated by the arrow. B, domain structure of human PRMT7
compared with that of TbPRMT7. Four closely spaced vertical lines indicate
AdoMet binding (or AdoMet binding-like, see text) domains; single vertical
line indicates “THW” loops. The degree of homology between
TbPRMT7 and human PRMT7 in the most conserved region (spanning 248 amino
acids; indicated by the horizontal line) is shown above the diagram.
The percent identity and similarity in this region is indicated. C,
ClustalW alignment of TbPRMT7 with the N-terminal methyltransferase motifs of
PRMT7 homologues from humans (HsPRMT7N (GenBank™ accession number
NP_061896)) and Drosophila (DmDart7N (GenBank™ accession number
NP_611753)). White letters on black background, identical;
white letters on gray background, conserved.
Asterisks indicate the conserved glutamate residues of the double E
loop.