FIGURE 4.

TbPRMT7 is expressed in two life cycle stages and its depletion does not affect growth. A, qRT-PCR analysis of the relative amounts of TbPRMT7 mRNA in procyclic (PF) and bloodstream (BF) form T. brucei. TbPRMT7 levels were normalized to β-tubulin and RNA levels in PF were set to one. B, clonal procyclic (upper) and bloodstream (lower) form T. brucei cell lines expressing tetracycline-regulated TbPRMT7 RNAi were established. Production of dsRNA was induced using 2.5 μg/ml tetracycline on Day 0, and total cells were counted thereafter until day 10 (PF) or 12 (BF). Depletion of TbPRMT7 mRNA was confirmed using qRT-PCR (PF) or Northern blotting (BF).