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Interaction of CIP85 with Cx43 in vitro. Lysates from bacteria expressing Flag-CIP85 or the vector alone were incubated with or without lysates containing HA-Cx43 (lanes 1−3). Flag-CIP85 complexes were collected on glutathione-agarose beads and immunoblotted with a Cx43 antibody. Lysates containing either Flag-CIP85 (lane 4) or HA-Cx43 (lane 5) were used as positive controls.
