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. Author manuscript; available in PMC: 2010 Apr 3.
Published in final edited form as: J Mol Biol. 2009 Feb 9;387(3):607–618. doi: 10.1016/j.jmb.2009.02.001

Figure 2.

Figure 2

gp13 activity on purified B. subtilis peptidoglycan. Mutanolysin digestion of B. subtilis sacculi produces a characteristic profile of peaks (blue line). The untreated sacculi (blue) show the characteristic pattern of cross-linked (>60 min) and uncross-linked (<40 min) muropeptides. Following incubation with purified gp13 protein a modest increase in monomer disaccharide (DS) tripeptide (TriP) and DS tetrapeptide (TP) peaks (under 40 min) is apparent with a slight decrease in cross-linked peaks beyond 60 min (red line). The 43.5 min peak present in the gp13 sample (* red line) is a contaminant from the purified protein solution.