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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Feb 21;1792(4):248–259. doi: 10.1016/j.bbadis.2009.02.007

Figure 3. Selection Process to Establish Panc-1 Subclones.

Figure 3

(A) Panc-1 cells are plated by limiting dilution into a 96-well plate and cultured for two weeks at which time clones were selected based on a ductal or transformed phenotype. Characterization of Panc-1 Subclones. Panc-1 clones (B) B10 tumorigenic (ductal), (C) C4 tumorigenic/mildly metastatic (ductal), (D) C5 tumorigenic/highly metastatic (transformed), (E) F9 tumorigenic/highly metastatic (transformed) were analyzed by FACS for their SP content.