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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Feb 21;1792(4):248–259. doi: 10.1016/j.bbadis.2009.02.007

Figure 5. Enrichment of SP by serial sub-culture in vitro.

Figure 5

The ‘side population’ assay. Live cells are stained with Hoechst 33342 dye, gated for live cells, and dual emission wavelengths from the dye are plotted on a log scale. The ‘R1’ gate designates the ‘side population’ (SP). (A) SP fraction from Panc-1 cells make up 10% of the total population, were sorted onto dishes, (B) cultured for one week and reanalyzed, resulting in an increase of the SP fraction to 50%. Identification of SP within isolated MP. Panc-1 cells were sorted for SP and MP fractions, (C) resulting in 10% SP. MP cells from this sort were plated onto dishes. (D) SP analysis of MP the plated MP fraction after sub-culture for four weeks resulted in identification of 5% SP either by residual or by conversion.