Fig. 2.
Expression of the first (Rluc) and second (Fluc) reporters following transient transfection of pRL-RF/hTPH2–5′-UTR constructs into the HEK-293 (A), RN46A (B), SK-N-MC (C), HEK-293T (D), HELA (E) and PC12 (F) cells. The Fluc and Rluc activities (Means±SE) of the constructs are expressed as folds relative to the Fluc and Rluc activity of the control (pRL-RF without insert), respectively. The Fluc activity (%) relative to Rluc for each construct can be estimated as Fluc(fold)×Rc(%)/Rluc(fold), where Rc is the relative Fluc activity (% of Rluc) shown in Fig. 1B.