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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Gene. 2009 Jan 21;435(1-2):53–62. doi: 10.1016/j.gene.2008.12.019

Fig. 4.

Fig. 4

Detection of the reporter mRNA levels and transcripts in HEK-293T cells transiently transfected with specific pRL-RF/hTPH2–5′-UTR constructs. (A) Fluc/Rluc mRNA ratio in HEK-293T cells; (B) Northern blot analysis using the antisense Fluc probe (complementary to sense Fluc); (C) Illustration of the mRNA transcripts derived from the pRL-RF/hTPH2–5′-UTR constructs; (D) Northern blot analysis using the sense Rluc probe. Levels of the reporter mRNA ratio are shown in Means±SE. The overall F=35.79 (P<0.0001) for ANOVA post-hoc test. *P<0.05, ***P<0.001 compared between the indicated constructs, ###P<0.001 compared with the constructs containing hTPH2 5′-UTR insert. Two sense transcripts (R1 and R2) reactive to antisense Fluc probe, as well as two antisense transcripts (R3 and R4) reactive to sense Rluc probe were observed. The sense transcript R2 is introduced by the insertion of hTPH2 5′-UTR with an expected size, and its abundance parallels the mRNA and activity (protein) levels of Fluc.