Table 1.

Primers used for the cloning and quantitative RT-PCR.

Name	Sequence
Cloning
NotI-pGL(7)F	5′-ttctGCGGCCGCactggccggtacctgagctc-3′
BamHI-pGL(2031)R	5′-cgcaaacGGATCCttatcgat-3′
SacI-hTPH2(1)F	5′-tgagGAGCTCcattgctcttcagcaccagg-3′
SacI-hTPH2(61)F	5′-atcgGAGCTCccttcctctcaatctccg-3′
SacI-hTPH2(99)F	5′-tactGAGCTCctagtaccccctgctgca-3′
XhoI-hTPH2(53)R	5′-agagCTCGAGgatcagctgcctgcttgg-3′
XhoI-hTPH2(78)R	5′-gcagCTCGAGcggagattgagaggaagg-3′
XhoI-hTPH2(116)R	5′-attcCTCGAGtgcagcagggggtactag-3′
XhoI-hTPH2(141)R	5′-ctggCTCGAGggatcccggtgtaatattctttc-3′
XhoI-hTPH2(1)F	5′-tgagCTCGAGcattgctcttcagcaccagg-3′
SacI-hTPH2(141)R	5′-ctggGAGCTCggatcccggtgtaatattctttc-3′
hTPH2(117)F*	5′-CgagaaagaatattacaccgggatccC-3′
hTPH2(141)R*	5′-TCGAGggatcccggtgtaatattctttctcGAGCT-3′
qRT-PCR
Rluc(735)F	5′-gataactggtccgcagtggt-3′
Rluc(957)R	5′-accagatttgcctgatttgc-3′
Fluc(594)F	5′-caccttcgtgacttcccatt-3′
Fluc(761)R	5′-tgactgaatcggacacaagc-3′

^*5′-phosphorylated linker. Upper case - designed to introduce the restriction site. Numbers in the parentheses represent the starting positions in target sequences (hTPH2, pGL4.14, and pRL-SV40). Positions in hTPH2 are relative to the transcription start site (TSS), while those in the reporter vectors are based on the sequences of pGL4.14 (AY864928, Fluc) and pRL-SV40 (AF025845, Rluc), respectively.