Table 1.

Gel mobility ratio and p53-induced DNA bending and twisting

p53	Ratio, r	Bend1, °	Bend2, °	ΔTwist, °
p53DBD	0.81	32.0	36.0	≈35
wt p53	0.71	50.5	56.5	≈70

Ratio, r, is the value of relative mobility shown in Fig. 2C for the spacer of 40 bp (minimum mobility of the complex divided by maximum mobility). The averages for three independent measurements are given, with the standard errors not exceeding 0.02. The bend angles are calculated following Eq. 2 assuming that the DNA bending per A₆ tract is 18° (bend₁) or 20° (bend₂) (see ref. 28). The changes in DNA twisting, ΔTwist, are deduced from Figs. 3 C and D.