FIG. 4.

Requirement of an intact alternative pathway for antimannan Fab M1-mediated C3b deposition to C. albicans yeast cells. Kinetics of C3b deposition from 2 to 10 min were determined in yeast-absorbed normal human serum depleted of factor B, C2, or C1q in the absence of antimannan antibody or in the presence of M1 or M1g1 at 10 μg per ml. Cell-bound C3 was quantified by use of FITC-anti-C3 antibody and flow cytometry. A representative experiment from two is shown.