CtsB inhibition in CCl4-treated mice. (A) Experimental setup of CCl4 and Ca074Me administration to mice. (B-F) 6-week after initial CCl4 challenge we determined serum ALT levels (B), H&E staining of liver sections (C), hydroxyproline levels in liver homogenate (D), Sirius Red staining of liver sections(E), and immunostaining with α-SMA and myeloperoxidase (MPO) to evaluate neutrophilic infiltration (F) in the different groups. Data are mean±SEM, in B, n=5 animals per group and *p≤ 0.01 vs. corn oil-treated mice; in D, n=5 animals per group and *p≤ 0.01 vs. corn oil-treated mice, and #p≤ 0.05 vs. CCl4+vehicle-treated mice.