Fig. 5.

Extrapituitary expression of luciferase in BAC PRL-Luc transgenic rat. A, In vivo imaging of vehicle (V) and LPS-treated rats. The rats were injected ip (arrows indicate site of injection) either with physiological saline or with 3 mg/ml LPS and imaged 16 h after the treatment. The images were acquired with an IVIS Spectrum (Caliper Life Sciences): 30 sec integration time, Bin (HR) 8, FOV 19.6, f1. B, RT-PCR strategy for the analysis of luciferase expression driven by the extrapituitary promoter. Dotted line, Observed alternative splicing. C, RT-PCR result. M, 100-bp ladder; T, thymus; SP, spleen.