Table 1.
Quantification of P. gingivalis colocalization with lipid rafts in J774A.1 macrophages a
| Cell condition | % Colocalization b |
|---|---|
| Untreated | 76.7 ± 13.3 |
| Cholesterol depleted c | 13.7 ± 6.2 * |
| Cholesterol reconstituted d | 67.3 ± 13.1 |
| Impaired phagocytosis e | 70.1 ± 19.9 |
a
The colocalization of FITC-P. gingivalis 33277 with Alexa Fluor 594-CTB—stained GM1(lipid raft marker) was quantified using ImageJ intensity correlation analysis. See Fig. 2 legend for experimental details.
b
Means ± SD of 15–20 examined cells from three independent experiments.
c
By pretreatment with 10 mM MCD.
d
By addition of 1 mM cholesterol to MCD-pretreated cells.
e
By pretreatment with 5 μg/ml cytochalasin D.
*
p < 0.05 compared to all other conditions.