Figure 6.
Quantification of the progeny of Pax6 knock-down cells. (A) LeX+ cells cultured in the expansion medium (n = 3). 3 div after transfection with either control (cRNA) or siRNA Pax6, double-labeling experiments show the same number of β-III-tubulin+ neurons and GFAP+ astroglia regardless of transfection. (B) When these cells were kept in differentiation medium for 5 div more neurons are generated from control (cRNA) than from siRNA transfected cells (*P < 0.05). In contrast, more GFAP cells were generated from siRNA transfected RG cells (P < 0.05).
