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. 1999 Mar 2;96(5):1881–1885. doi: 10.1073/pnas.96.5.1881

Figure 1.

Figure 1

Protein immunoblots showing expression of the Gal4 DNA-BD-5LO (Left) and the Gal4 AD-5LO-interacting protein fusions (Right) in the yeast strain PJ69–4A. Yeast protein extracts were fractionated by 7.5% or 10% SDS/PAGE and subjected to immunoblot (IB) analysis with anti-5LO (IB Anti-5LO) or anti-activating domain (IB Anti-AD) antibody, respectively. Purified 5LO (100 ng) was included as a control. The proteins were visualized by using alkaline phosphatase substrates (nitro blue tetrazolium and 5-bromo-4-chloro-3-indolylphosphate).