Skip to main content
. 2009 Apr 28;4(4):e5351. doi: 10.1371/journal.pone.0005351

Figure 7. Effect of doxycycline (DOX) on aggregate formation and heart weight in tTA/HSP B8/R120G triple TG mice (Triple TG) at 30 weeks of age.

Figure 7

DOX was administered via drinking water (1g/l) from 4 weeks of age. (A) Representative Western blots using hearts from tTA/HSPB8 double transgenic (tTA/HSPB8 TG) mice with DOX, HSPB5 R120G transgenic (R120G TG) and tTA/HSPB8/HSPB5 R120G triple transgenic (Triple TG) mice with DOX. (B) Quantitative analysis of HSPB8 levels (n = 4 mice). Values are the -fold increase relative to those in non-transgenic (NTG) mice whose values are arbitrarily set to 1. (C) Representative pictures of the immunohistochemistry are shown. Green color shows HSPB5 and red color shows troponin I. (D) Typical picture of the filter assay for the detection of the aggregates. The aggregates containing the mutant HSPB5 R120G protein are observed in R120G TG mice (No. 4) and triple TG mice with doxycycline (No. 5). (E) Quantitative analysis of aggregates (n = 6 mice). Values are the -fold increase relative to those in NTG mice (No.1) whose values are arbitrarily set to 1. (F) The ratios of heart weight per body weight (n = 6 mice). Cardiac enlargement is seen in R120G TG mice (No. 4) and triple TG mice (No. 5) whereas no difference is observed among NTG (No.1), tTA/HSPB8 TG (No. 2) or tTA/HSPB8 mice with DOX. *** p<0.001 vs. NTG mice; ### p<0.001 vs. tTA/HSPB8 TG mice; aaa p<0.001 vs. R120G TG mice; bb p<0.01 vs. Triple TG mice.