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. 2009 May 1;5(5):e1000411. doi: 10.1371/journal.ppat.1000411

Figure 2. Adherence of S. epidermidis surface molecules to explanted DLs.

Figure 2

(A) Cell wall–associated SdrF mediates L. lactis cells adherence to explanted DLs. PIA production by L. lactis elicits lower levels of adherence than SdrF. A.U.A., arbitrary units of adherence; *, P<0.05 when compared to pOri23 (control); §, P<0.05 when compared to SdrF. Recombinant phenotype analyzed by flow cytometry is shown below. A.U.F., arbitrary units of fluorescence calculated by multiplying the percentage of positive (fluorescent) events by the average fluorescence per event. #, normalized A.U.F. was calculated assigning an arbitrary value of 1.0 to the control strain (pOri23) incubated with the appropriate polyclonal antibody for each recombinant protein. &, PIA phenotype was determined by biofilm production assessment as previously described [23]. (B) Gram stain view (1000×) of L. lactis (pOri-SdrF) cells adherent to an explanted DL. DF, Dacron fibers; Cn, collagen fibers; LL, L. lactis cells.